Effect of astragalus polysaccharide on the function and maturation of plasmacytoid dendritic cells from chronic myelogenous leukemia before and after treatment / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 740-743, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-353557
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of astragalus polysaccharide (APS) on the function and maturation of chronic myelogenous leukemia (CML) peripheral blood mononuclear cells (PBMC)-derived plasmacytoid dendritic cells (pDCs).</p><p><b>METHODS</b>CML-derived pDCs were sorted by flow cytometry, and then incubated with APS (at 0, 50, 100 and 200 mg/L). After 24 hours, the concentrations of IFN-α, IL-6, TNF-α were detected with ELISA. Five days later, the cultured cells were collected and analyzed for immotype, morphology and ultramicrostructure.</p><p><b>RESULTS</b>The level of IFN-α, IL-6, TNF-α was significantly higher in samples from CML remission group than that in untreated pDCs, and newly diagnosed pDC (P < 0.05) or untreated group. APS could promote more pDCs differentiating to dendritic cells (DCs) in CML remission group than in untreated-pDCs in a dose-dependant manner (P < 0.05).</p><p><b>CONCLUSION</b>APS can enhance the immune function of pDCs, promote differentiation and maturation of pDCs from CML patients.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Polysaccharides
/
Dendritic Cells
/
Leukocytes, Mononuclear
/
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
/
Cells, Cultured
/
Interferon-alpha
/
Allergy and Immunology
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2010
Type:
Article
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