Significance of detection of anti-GPIIb/IIIa antibody secreting B cells and platelet-specific antibody in patients with idiopathic thrombocytopenic purpura / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 603-606, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-353608
ABSTRACT
<p><b>OBJECTIVE</b>To detect the frequencies of anti-GPIIb/IIIa antibody secreting B cells and platelet-specific antibody in patients with idiopathic thrombocytopenic purpura (ITP) and non-immune thrombocytopenia, and to evaluate their roles in the diagnosis of ITP and their clinical significance.</p><p><b>METHODS</b>The frequencies of circulating B cells secreting anti-GPIIb/IIIa antibody and platelet-specific antibody in 58 ITP patients, 33 non-ITP patients and 31 healthy controls were tested by Enzyme-linked Immunospot Assay (ELISPOT) and modified monoclonal antibody immobilization of platelet antigens assay (MAIPA) respectively.</p><p><b>RESULTS</b>The frequencies of circulating B cells secreting anti-GPIIb/IIIa antibody in ITP patients \[(6.6 ± 4.2)/10(5) PBMNC\] were significantly increased (P < 0.05) than that of the controls \[(1.3 ± 0.5)/10(5) PBMNC\] and non-immune thrombocytopenic purpura patients \[(2.2 ± 2.0)/10(5) PBMNC\]. However there was no apparent difference between the latter two groups (P > 0.05). ELISPOT had a sensitivity of 70.69%, a specificity of 90.91% for the diagnosis of ITP, the sensitivity being higher than that of modified MAIPA's (43.10%) (χ(2) = 7.03, P < 0.05). The ROC curve showed the discriminative validity of cytometric bead array was 0.886.</p><p><b>CONCLUSION</b>The frequencies of circulating B cells secreting anti-GPIIb/IIIa antibody may reflect the pathogenesis of ITP. ELISPOT assay have high sensitivity and specificity than modified MAIPA for the diagnosis of ITP and the guidance for clinical therapy.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Autoantibodies
/
Blood Platelets
/
B-Lymphocytes
/
Purpura, Thrombocytopenic, Idiopathic
/
Platelet Glycoprotein GPIIb-IIIa Complex
/
Allergy and Immunology
Type of study:
Diagnostic study
/
Practice guideline
/
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2010
Type:
Article
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