Inhibitory effect of 2'-o-methoxyethyl-modified antisense oligonucleotides targeting vascular endothelial growth factor A on SKOV3 human ovarian cancer cells / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1573-1575, 2011.
Article
in English
| WPRIM
| ID: wpr-353942
ABSTRACT
<p><b>BACKGROUND</b>Ovarian cancers are often at an advanced stage at diagnosis because early detection is difficult. The poor prognosis of ovarian cancers highlights the crucial need to develop better therapeutic agents and strategies. The objective of this study was to investigate the inhibitory effects of a new modified antisense oligonucleotides targeting vascular endothelial growth factor A (VEGF-A) in SKOV3 ovarian cancer cells.</p><p><b>METHODS</b>Antisense oligonucleotides targeting VEGF-A was designed, synthesized and transfected into SKOV3 ovarian cancer cells. Western blotting and real-time RT-PCR were used to analyze the inhibitory effects of antisense oligonucleotides on VEGF-A protein and mRNA expression. Transwell matrix assay was used to detect cell migration inhibition.</p><p><b>RESULTS</b>The antisense oligonucleotides targeting VEGF-A significantly decreased VEGF-A protein and mRNA expression and inhibited cell migration in SKOV3 ovarian cancer cells.</p><p><b>CONCLUSIONS</b>This new modified antisense oligonucleotides targeting VEGF-A can decrease VEGF-A expression and inhibit cell migration in SKOV3 ovarian cancer cells. This new oligonucleotides may be a promising therapeutic agent for ovarian cancers.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovarian Neoplasms
/
Pharmacology
/
Cell Movement
/
Blotting, Western
/
Oligonucleotides, Antisense
/
Cell Line, Tumor
/
Vascular Endothelial Growth Factor A
/
Metabolism
Type of study:
Prognostic study
/
Screening study
Limits:
Female
/
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2011
Type:
Article
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