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Microarray profiles on age-related genes in the earlier postnatal rat visual cortex / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 1545-1550, 2011.
Article in English | WPRIM | ID: wpr-353947
ABSTRACT
<p><b>BACKGROUND</b>Accumulating evidence indicates that both innate and adaptive mechanisms are responsible for the postnatal development of the mammalian visual cortex. Most of the studies, including gene expression analysis, were performed on the visual cortex during the critical period; few efforts were made to elucidate the molecular changes in the visual cortex during much earlier postnatal stages. The current study aimed to gain a general insight into the molecular mechanisms in the developmental process of the rat visual cortex using microarray to display the gene expression profiles of the visual cortex on postnatal days.</p><p><b>METHODS</b>All age-matched Sprague-Dawley rats in various groups including postnatal day 0 (P0, n = 20), day 10 (P10, n = 15), day 20 (P20, n = 15) and day 45 (P45, n = 10) were sacrificed respectively. Fresh visual cortex from the binocular area (Area 17) was dissected for extraction of total RNA for microarray analyses. Taking advantage of annotation information from the gene ontology and pathway database, the gene expression profiles were systematically and globally analyzed.</p><p><b>RESULTS</b>Of the 31 042 gene sequences represented on the rat expression microarray, more than 4000 of the transcripts significantly altered at days 45, 20 or 10 compared to day 0. The most obvious alteration of gene expression occurred in the first ten days of the postnatal period and the genomic activities of the visual cortex maintained a high level from birth to day 45. Compared to the gene expression at birth, there were 2630 changed transcripts that shared in three postnatal periods. The up-regulated genes in most signaling pathways were more than those of the down-regulated genes.</p><p><b>CONCLUSIONS</b>Analyzing gene expression patterns, we provide a detailed insight into the molecular organization of the developing visual cortex in the earlier postnatal rat. The most obvious alteration of gene expression in visual cortex occurred in the first ten days. Our data were a basis to identify new relevant candidate genes that control visual cortex development.</p>
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Visual Cortex / Gene Expression Regulation, Developmental / Oligonucleotide Array Sequence Analysis / Gene Expression Profiling / Genetics / Metabolism Limits: Animals Language: English Journal: Chinese Medical Journal Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Visual Cortex / Gene Expression Regulation, Developmental / Oligonucleotide Array Sequence Analysis / Gene Expression Profiling / Genetics / Metabolism Limits: Animals Language: English Journal: Chinese Medical Journal Year: 2011 Type: Article