Effect of curettage and copper wire on rabbit endometrium: a novel rabbit model of endometrial mechanical injury / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1708-1713, 2011.
Article
in English
| WPRIM
| ID: wpr-353979
ABSTRACT
<p><b>BACKGROUND</b>It remains almost a helpless situation for the recurrent implantation failure and pregnancy loss caused by endometrial injury at present. The purpose of this study was to develop a rabbit model of endometrial mechanical injury that could provide a research platform for this difficult clinical predicament.</p><p><b>METHODS</b>Three experiments were conducted. Experiment 1 Curettages in both uterus horns and copper wire inserting after curettage (double-injury) in one horn. The histological changes were monitored at 0, 24, 48, 72 hours, as well as in 1 and 2 weeks after operation. Experiment 2 Direct copper wire inserting in one horn and double-injury in other horn. The wires in both horns were removed after 2 weeks. The histological changes were recorded at 0, 1 and 2 weeks after wire removal. Experiment 3 Double-injury procedure in one horn was performed and wire was removed after 2 weeks; another horn was remained normal to serve as control. Histological changes were recorded, tissue areas were measured, and proliferation indices (PIs, %) were calculated at 1, 2, 4 and 8 weeks after wire removal, respectively.</p><p><b>RESULTS</b>The experiments revealed that the injured endometrium by simple curettage or copper wire could be fully repaired. While the endometrial regeneration was severely impaired by double-injury, both areas of endometrium and uterine cavity decreased (P < 0.05); both PIs of glandular epithelial and stromal cells increased and reached maximum at 4 weeks (P < 0.05), but returned by 8 weeks.</p><p><b>CONCLUSION</b>This study demonstrated that a rabbit model of endometrial injury could be effectively established through a double-injury procedure of curettage and copper wire with comparable clinical index.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Wounds and Injuries
/
Immunohistochemistry
/
Copper
/
Curettage
/
Disease Models, Animal
/
Endometrium
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2011
Type:
Article
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