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Anticancer activities of curcumin on human Burkitt's lymphoma / 中华肿瘤杂志
Zhonghua zhong liu za zhi ; (12): 348-352, 2002.
Article in En | WPRIM | ID: wpr-354026
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the anticancer activities of curcumin on human Burkitt's lymphoma and their molecular mechanism.</p><p><b>METHODS</b>The effect of curcumin on the growth of CA46 cells and apoptosis were studied through Trypan blue exclusion, MTT assay, cell cycle, DNA fragmentation analysis and detection of TdT-mediated dUTP nick end labeling (TUNEL). The effect of curcumin on the expression of c-myc, bcl-2, mutant-type p53 and Fas protein and mRNA was studied by flow cytometry (FCM) and reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>1. Curcumin inhibited proliferation of CA46 cells in a time- and dose-dependent manner, 2. CA46 cells treated with curcumin showed G(0)/G(1) or G(2)/M phase increase and S phase decrease, 3. CA46 cells apoptosis induced by curcumin was confirmed by DNA fragmentation and TUNEL and 4. The expression of c-myc, bcl-2, mutant-type p53 protein and mRNA was decreased sharply in CA46 cells treated with curcumin, while Fas protein and mRNA was increased.</p><p><b>CONCLUSION</b>Curcumin is able to inhibit the proliferation of CA46 cells and induce the cell apoptosis by down-regulating the expression of c-myc, bcl-2, mutant-type p53 and up-regulating the expression of Fas.</p>
Subject(s)
Full text: 1 Index: WPRIM Main subject: Pharmacology / Tumor Cells, Cultured / Cell Cycle / Cell Division / Gene Expression Regulation / Tumor Suppressor Protein p53 / Proto-Oncogene Proteins c-myc / Burkitt Lymphoma / Apoptosis / Fas Receptor Limits: Humans Language: En Journal: Zhonghua zhong liu za zhi Year: 2002 Type: Article
Full text: 1 Index: WPRIM Main subject: Pharmacology / Tumor Cells, Cultured / Cell Cycle / Cell Division / Gene Expression Regulation / Tumor Suppressor Protein p53 / Proto-Oncogene Proteins c-myc / Burkitt Lymphoma / Apoptosis / Fas Receptor Limits: Humans Language: En Journal: Zhonghua zhong liu za zhi Year: 2002 Type: Article