Study on methodology of determination of Yizhi osmotic pump tablets active ingredient in Beagle dog plasma by LC-MS/MS / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1964-1968, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-354152
ABSTRACT
<p><b>OBJECTIVE</b>To develop a sensitive and specific LC-MS/MS method for determination of Yizhi osmotic pump tablets active ingredient in Beagle dog plasma.</p><p><b>METHOD</b>Beagle dog plasma pre-treatment methods were established. Geniposide, notoginsenoside R1, ginsenoside Rg1 and Rb1 notoginsenoside molecular ions and fragment ions peaks were separated well and detected synchronously by LC-MS/MS with digoxin as internal standard.</p><p><b>RESULT</b>Under the selected LC-MS/MS conditions, the characteristic fragment ions of the four components could be well separated and quantified, and the calibration curves showed good linearity within a certain concentration range of each component; extraction recoveries of those four compounds in plasma were higher than 75%, method recoveries were higher than 90%; day precision (RSD <10%) and inter-day precision (RSD <15%) were generally fine; freeze-thaw and room temperature experiments showed good stability (RSD <15%).</p><p><b>CONCLUSION</b>The method is proved to be suitable for pharmacokinetic studies of Yizhi active ingredients in Beagle dog plasma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osmosis
/
Tablets
/
Temperature
/
Time Factors
/
Blood Chemical Analysis
/
Calibration
/
Drugs, Chinese Herbal
/
Pharmacokinetics
/
Chromatography, Liquid
/
Drug Stability
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article
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