Real-time detection of mast cell degranulation in anaphylactoid reaction / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1860-1864, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-354173
ABSTRACT
<p><b>OBJECTIVE</b>To establish a new, real time, dynamic and direct optical detection method for mast cell degranulation caused by anaphylactoid reaction.</p><p><b>METHOD</b>A CD63-GFP plasmid was constructed and introduced steadily into rat basophilic leukemia (RBL-2H3) cells. The movements of CD63-GFP, which was located on both the granule membranes and the plasma membranes of RBL cells stimulated by Compound 48/80, were studied by confocal laser scanning microscope (CLSM) and total internal reflection fluorescence microscope (TIRFM) both inside and on the surface of living RBL-2H3 cells.</p><p><b>RESULT</b>Before antigen stimulation, most granules with CD63-GFP hardly moved in RBL cells. However, after antigen stimulation, the granules moved dramatically. They reached the plasma membranes in a few minutes and fused with them instantaneously. The velocity of the granule movement toward the plasma membranes on antigen stimulation was calculated to be 0.05 micron x s(-1).</p><p><b>CONCLUSION</b>Analysis of the movement of each granule provided a new insight into the elementary process of degranulation. The method is rapid, sensitive and reliable, which could be used as a new detection method for anaphylactoid reaction in vitro.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Platelet Membrane Glycoproteins
/
Antigens, CD
/
Cell Degranulation
/
Cell Movement
/
Microscopy, Confocal
/
Cell Biology
/
Cell Line, Tumor
/
Diagnosis
/
Allergy and Immunology
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article
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