Effect of ultrafiltration by different membranes on HPLC fingerprint of huanglian jiedu decorction after being / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the change of HPLC fingerprint of Huanglian Jiedu decroction. Huanglian Jiedu decorction was ultrafiltrated by different membranes.</p><p><b>METHOD</b>Different relative molecular weight cutoff membranes were used (pes-5w, pes-3w, pes-2w, pes-1w, pes-6k) to explore its effects on content differences among all samples in HPLC. The turbidity, viscosity and other physical and chemical parameters were measured.</p><p><b>RESULT</b>Berberine, baicalein and jatrorrhizine became smaller while geniposide increased. Physicall and chemical parameters also showed regular changes. Taking centrifugate as reference, the similarities of the samples treated with high to low molecular weight cutoff membranes were 0.993, 0.992, 0.984, 0.982, 0.975, respectively.</p><p><b>CONCLUSION</b>In this study, the ultrafiltration was used, HPLC fingerprint showed the differences of the samples, and the change of the physical and chemical parameters was explored.</p>