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Glossy ganoderma spore oil promotes apoptosis of human lung adenocarcinoma SPC-A1 through downregulation of miR-21 / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 1231-1234, 2011.
Article in Chinese | WPRIM | ID: wpr-354211
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of glossy ganoderma spore oil on the proliferation, apoptosis, expression of miR-21 and its target genes of human lung adenocarcinoma SPC-A1 cell line, and to explore its possible mechanism.</p><p><b>METHOD</b>The SPC-A1 cells were treated with glossy ganoderma spore oil for 24 and 48 hours. The inhibition growth efficacy was determined using cell count kit (CCK-8). Cell morphological changes were observed by light microscopy. Cell apoptosis was analyzed by flow cytometry. The expression of miR-21, PTEN and PDCD4 were determined by Real-time PCR.</p><p><b>RESULT</b>Glossy ganoderma spore oil concentration-dependently inhibited the SPC-A1 cell's proliferation. When the concentration of glossy ganoderma spore oil attained to 0.2%, the cells' morphology changed obviously. Glossy ganoderma spore oil could induce the apoptosis of SPC-A1 cells at low concentration. Glossy ganoderma spore oil down-regulated the expression of miR-21 and up-regulated the expression of PTEN and PDCD4 significantly.</p><p><b>CONCLUSION</b>glossy ganoderma spore oil could inhibit the proliferation obviously and cause the changes of cell morphology. Furthermore, glossy ganoderma spore oil induced apoptosis of SPC-A1 cell through down-regulating the expression of miR-21 and up-regulating tumor suppressors.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Spores, Fungal / Adenocarcinoma / Chemistry / Polymerase Chain Reaction / Apoptosis / Ganoderma / MicroRNAs / Cell Line, Tumor / Genetics Limits: Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Spores, Fungal / Adenocarcinoma / Chemistry / Polymerase Chain Reaction / Apoptosis / Ganoderma / MicroRNAs / Cell Line, Tumor / Genetics Limits: Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2011 Type: Article