Production of polyclonal antibody against HBD-2 by immunization with recombinant GST-HBD-2 fusion protein / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 575-579, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-354247
ABSTRACT
For the purpose of detecting the HBD-2 expression at protein level, the recombinant prokaryotic expression vector pGEX-1lambdaT-HBD-2 was constructed and the E. coli-based product of GST-HBD-2 fusion protein was prepared. When rabbit was immunized with the fusion protein, the anti-serum against HBD-2 was produced. After caprylic acid and ammonium sulfate precipitation, high titer of specific polyclonal antibody against HBD-2, which was detected by ELISA and Western blot, was obtained. This result suggests that recombinant peptide fusion protein could be used instead of the conjugate of peptide-albumin or peptide-thyroid globulin to produce antibody. The obtained antibodies could be used for revealing the tissue distribution of HBD-2 and the regulation of its gene expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Fusion Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Blotting, Western
/
Immunization
/
Beta-Defensins
/
Allergy and Immunology
/
Escherichia coli
/
Metabolism
/
Antibodies
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS