Effect of nm23-H1 on reversing malignant phenotype on human lung cancer cell line L9981 / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 530-534, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-354257
ABSTRACT
nm23-H1 is a proven tumor metastasis suppressive gene, tumor metastasis phenotype could be reversed by transfected nm23-H1 cDNA. This study was conducted to transfect nm23-H1 cDNA into L9981 cells and to explore the function of nm23-H1 in reversing the malignant phenotype of L9981 cells. The plasmid of pLXSN-nm23-H1-EGFP was constructed by gene clone technique, and the transfected nm23-H1 cDNA cell lines of L9981-nm23-H1 was established. The protein expression of nm23-H1 was detected by Western blot. The biologic features of L9981-nm23-H1 cells were studied in vitro and in vivo. The results showed that the fusion protein of nm23-H1-EGFP was stable, continuous and expressed with high efficiency in L9981-nm23-H1 cells. The cell proliferation, colon formation and invasive ability are significantly lowered in L9981 cells transfected nm23-H1 cDNA (P < 0.01); the tumorgenesis and the lung metastasis incidence was lower in tranfected nm23-H1 cells than in L9981 and L9981-Plxsn in nude mice (P < 0.01); the rate for inhibiting tumorgenesis of nm23 -H1 was 82.56%. These data suggest that the malignant phenotype could be reversed by wild nm23-H1 gene in L9981 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Recombinant Fusion Proteins
/
Transfection
/
Tumor Suppressor Proteins
/
Cell Line, Tumor
/
Cell Proliferation
/
NM23 Nucleoside Diphosphate Kinases
/
Genetics
/
Lung Neoplasms
/
Mice, Nude
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2005
Type:
Article
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