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Effect of nm23-H1 on reversing malignant phenotype on human lung cancer cell line L9981 / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 530-534, 2005.
Article in Chinese | WPRIM | ID: wpr-354257
ABSTRACT
nm23-H1 is a proven tumor metastasis suppressive gene, tumor metastasis phenotype could be reversed by transfected nm23-H1 cDNA. This study was conducted to transfect nm23-H1 cDNA into L9981 cells and to explore the function of nm23-H1 in reversing the malignant phenotype of L9981 cells. The plasmid of pLXSN-nm23-H1-EGFP was constructed by gene clone technique, and the transfected nm23-H1 cDNA cell lines of L9981-nm23-H1 was established. The protein expression of nm23-H1 was detected by Western blot. The biologic features of L9981-nm23-H1 cells were studied in vitro and in vivo. The results showed that the fusion protein of nm23-H1-EGFP was stable, continuous and expressed with high efficiency in L9981-nm23-H1 cells. The cell proliferation, colon formation and invasive ability are significantly lowered in L9981 cells transfected nm23-H1 cDNA (P < 0.01); the tumorgenesis and the lung metastasis incidence was lower in tranfected nm23-H1 cells than in L9981 and L9981-Plxsn in nude mice (P < 0.01); the rate for inhibiting tumorgenesis of nm23 -H1 was 82.56%. These data suggest that the malignant phenotype could be reversed by wild nm23-H1 gene in L9981 cells.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Recombinant Fusion Proteins / Transfection / Tumor Suppressor Proteins / Cell Line, Tumor / Cell Proliferation / NM23 Nucleoside Diphosphate Kinases / Genetics / Lung Neoplasms / Mice, Nude Limits: Animals / Humans Language: Chinese Journal: Journal of Biomedical Engineering Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Recombinant Fusion Proteins / Transfection / Tumor Suppressor Proteins / Cell Line, Tumor / Cell Proliferation / NM23 Nucleoside Diphosphate Kinases / Genetics / Lung Neoplasms / Mice, Nude Limits: Animals / Humans Language: Chinese Journal: Journal of Biomedical Engineering Year: 2005 Type: Article