Primary research of reconstruction of complete biological tissue engineered blood vessel / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 448-451, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-354277
ABSTRACT
To explore primarily the reconstruction of biological tissue engineered blood vessel in vitro, the decellularised scaffolds were obtained from swine common carotid arteries by enzyme digestion, and vascular smooth muscle cells (VSMCs) were isolated from canine thoracic aorta and subcultured and purified. The VSMCs were seeded into the inner surface of scaffolds and they were cocultured in vitro for 4 weeks. Histological staining and transmission electron microscopy were used to observe the growth of canine VSMCs on swine decellularized arterial scaffolds. The results showed the seeded VSMCs grew well on the decellularized scaffold throughout the duration of 4 weeks, which suggests that the method in this article is practicable for reconstructing complete biological tissue engineering blood vessel.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Arteries
/
Swine
/
Blood Vessel Prosthesis
/
Cells, Cultured
/
Coculture Techniques
/
Cell Biology
/
Tissue Engineering
/
Myocytes, Smooth Muscle
/
Microscopy, Electron, Transmission
/
Tissue Scaffolds
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2005
Type:
Article
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