Relative quantification of cytokeratin 19 transcription in oral squamous cell carcinoma tissues by fluorescent quantitive real-time RT-PCR / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 553-555, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-354317
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relative quantification of cytokeratin 19 transcription in oral squamous cell carcinoma tissues by fluorescent quantitive real-time reverse transcription-polymerase chain reaction ((RT-PCR).</p><p><b>METHODS</b>CK19mRNA level was detected by fluorescent quantitive real-time RT-PCR in cancerous and para-cancerous tissues from 31 oral squamous cell carcinoma patients. According to the 2(-DeltaDeltaCt) equation, the relative quantification fold of CK19mRNA level was calculated in cancerous tissues compared with para-cancerous tissues.</p><p><b>RESULTS</b>CK19mRNA levels in cancerous tissues were 2.21 folds higher than those in para-cancerous tissues, and the amplicon was specific. CK19mRNA level in cancerous tissue correlated significantly with pathological differentiation degree, the poorer the differentiation was, the higher the CK19mRNA level became.</p><p><b>CONCLUSIONS</b>Fluorescent quantitive real-time RT-PCR is accurate and reliable in the detection of relative quantification of CK19 transcription in oral squamous cell carcinoma tissues.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Mouth Neoplasms
/
Carcinoma, Squamous Cell
/
Reverse Transcriptase Polymerase Chain Reaction
/
Keratin-19
/
Genetics
/
Metabolism
/
Methods
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2006
Type:
Article
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