Radiation-inducible promoters-mediated cdglytk gene in the treatment of buccal carcinoma in golden hamster / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 549-552, 2006.
Article
in Zh
| WPRIM
| ID: wpr-354318
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the therapeutic effect of CDglyTK gene mediated by radiation-inducible promoters in the treatment of buccal carcinoma in Golden Hamster.</p><p><b>METHODS</b>Animal models of buccal carcinoma in golden hamster were established by painting 0.5% dimethyl-benzanthracene. The plasmids pcDNA (+) 3.1/E-CDglyTK were transfected into tumors by lipofectamine. 24 h later, the tumors were exposed to 3 Gy irradiation. Animals were monitored at regular intervals for volume of tumors. CDglyTK mRNA was assayed by RT-PCR. Apoptosis and proliferating cell nuclear antigen were detected respectively by in situ end-labeling and immunohistochemical methods.</p><p><b>RESULTS</b>Compared with control groups, the tumor was suppressed obviously by CDglyTK gene therapy combined with 3 Gy induction radiation. The expression of CDglyTK gene could be detected by RT-PCR in the transfected tumor, and up-regulation of CDglyTK expression was found in tumor exposed to radiation (P < 0.05). There was significant difference in apoptosis index or proliferation index between tumor without irradiation and tumor with irradiation (P < 0.05).</p><p><b>CONCLUSIONS</b>The radiation-inducible promoter can be served as a molecular switch to regulate the expression of CDglyTK gene in buccal carcinoma in golden hamster, and low dose induction radiation can significantly improve the therapeutic effects.</p>
Full text:
1
Index:
WPRIM
Main subject:
Radiation Effects
/
Radiotherapy
/
Therapeutics
/
Thymidine Kinase
/
Mouth Neoplasms
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Diagnostic Imaging
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Carcinoma, Squamous Cell
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Radiography
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Genetic Therapy
/
Cheek
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Stomatology
Year:
2006
Type:
Article