An experimental study on rat bone marrow mesenchymal stem cells transfected with dentin sialophosphoprotein gene in vitro / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 426-429, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-354353
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the expression of dentin sialophosphoprotein (DSPP) in transfected rat bone marrow mesenchymal stem cells (BM-MSC) and the influence of the transfection.</p><p><b>METHODS</b>Plasmid containing mice dentin DSPP was constructed by using the cytomegalovirus (CMV) promoter and then transfected the cultured BM-MSC by lipofectamine; The expression of Pax-9 and dentin matrix protein 1 (DMP1) gene of transfected BM-MSC were detected by RT-PCR. The expression of DSPP was examined by immunocytochemical staining, and the formation ratio of mineralized nodules of transfected BM-MSC was compared with untransfected ones after mineralized induction.</p><p><b>RESULTS</b>The constructed pcDNA3.1(+)/mDSPP could produced 3.0 kb and 5.4 kb fragments, DSPP gene and Pax9 gene were expressed 24 h and 48 h respectively, after BM-MSC were transfected Pax-9 gene was exprssed, but DMP1 gene was not; Immunohistochemical staining showed that DSPP was positive in transfected BM-MSC; The formation ratio of mineralized nodules of transfected BM-MSC was higher than that of untransfected ones after mineralized induction.</p><p><b>CONCLUSIONS</b>The expression of mice DSPP in BM-MSC by gene transfection can induce the expression of tooth development-associated gene Pax9 and enhance the formation of mineralized nodules, which suggests that DSPP gene might induce odontogenic differentiation of BM-MSC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphoproteins
/
Protein Precursors
/
Sialoglycoproteins
/
Bone Marrow Cells
/
Transfection
/
Cells, Cultured
/
Extracellular Matrix Proteins
/
Mesenchymal Stem Cells
/
Genetic Vectors
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2006
Type:
Article
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