Effects of bone marrow mesenchymal stem cells on the proliferation of hepatocytes and cirrhotic fat-storing cells in vitro / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 681-684, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-354669
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differentiation of bone marrow mesenchymal stem cells (BMSCs) and the effects of BMSCs on the proliferation of cirrhotic fat-storing cells (CFSC) and hepatocytes in vitro.</p><p><b>METHODS</b>BMSCs and hepatocytes were isolated and harvested from the bone marrow and livers of rats. A co-culture system was set up by transwell inserts in which the two chambers were separated by a semipermeable membrane. BMSCs labeled with PKH26 were cultured with hepatocytes/CFSC in the co-culture system and also in a cell-cell direct contact culture system. Anti-albumin and anti-smooth muscle alpha-actin (alpha-SMA) antibodies were tested by using fluorescence immunocytochemistry. BMSCs and hepatocytes/CFSC cultured alone served as controls. The proliferation level of hepatocytes in the co-culture system was measured. CFSC were cultured with the conditional medium of BMSCs, and their quantities were measured microscopically.</p><p><b>RESULTS</b>Expression of albumin was observed in the hepatocytes of the two culture systems after they were cultured for 72 h but the albumin levels were higher in the cell-cell direct contact culture system (P<0.01). As compared to the controls, the number of hepatocytes was larger in the co-culture system (P<0.01). No expression of alpha-SMA in CFSC was observed in either culture system. The proliferation of CFSC was inhibited by the conditional medium of BMSCs. The longer the time of the co-culturing the more significant was the CFSC growth suppression (P<0.01).</p><p><b>CONCLUSIONS</b>BMSCs can be induced into hepatocytes by a local micro-environment formed by hepatocytes. BMSCs may promote proliferation of hepatocytes and inhibit proliferation of CFSC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bone Marrow Cells
/
Cell Differentiation
/
Cells, Cultured
/
Rats, Sprague-Dawley
/
Coculture Techniques
/
Hepatocytes
/
Cell Biology
/
Cell Proliferation
/
Mesenchymal Stem Cells
/
Liver Cirrhosis, Experimental
Limits:
Animals
/
Pregnancy
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2007
Type:
Article
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