Degradation of 14-3-3beta appeared in apoptosis cell induced by PrP106-126 polypeptide / 病毒学报
Chinese Journal of Virology
;
(6): 414-417, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-354715
ABSTRACT
To investigate changes of 14-3-3beta from apoptosis induced by PrP106-126 polypeptide, HeLa cell was incubated with PrP106-126 for 4h or 8h. Nucleus changes and the expression of PARP were detected differently by Hoechst staining and Western blotting. Expressing of protein and mRNA from 14-3-3beta was determined by Western blotting and Real-time PCR. The results show that typical nucleus pyknosis and chip of apoptosis and degradation of PARP were induced by PrP106-126 peptide in HeLa cells. Degradation of 14-3-3beta appeared in apoptosis groups induced by PrP106-126 peptide. However, 14-3-3beta mRNA did not display any changes in apoptosis groups. This study indicated that degradation of antiapoptosis protein 143-3beta induced by PrP106-126 peptide may be one of pathogenesis mechanism of prion disease.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptide Fragments
/
Pharmacology
/
Prions
/
HeLa Cells
/
Apoptosis
/
14-3-3 Proteins
/
Proteolysis
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2012
Type:
Article
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