Analyses on antigen epitopes and drug resistance mutations of HIV-1 gag and pol genes / 病毒学报
Chinese Journal of Virology
;
(6): 351-357, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-354724
ABSTRACT
To study the CTL antigen epitopes and drug resistance mutations of HIV-1 gag and pol genes through analyzing gag and pol gene sequences. The HIV-1 gag and pol gene fragments were amplified using nested polymerase chain reaction. A total of 23 PCR sequences, 449 cloned gag sequences and 402 cloned pol sequences were obtained. Sequence analyses showed the 23 samples were subtype B or B'. A total of 4 in 8 CTL antigen epitopes appeared 8 mutations in consensus sequence of subtype B and B'. There were no mutations found in the PCR sequences, whereas a few mutations were found in clone sequences (9.80%) in 5 antigen epitopes in p24 region. Eighteen PIs-related mutations and 24 RTIs-related mutations were found in PCR sequences and clone sequences in pol gene region, in which 17 (94.44%) PIs-related mutations and 15 (62.50%) RTIs-related mutations were found only in the clone sequences, respectively. The results showed that the prevalence of HIV-1 drug resistance strains in this study was at a higher level (17.39%), suggesting that some samples were resistant.to existing antiviral drugs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
DNA Mutational Analysis
/
T-Lymphocytes, Cytotoxic
/
HIV-1
/
Classification
/
Drug Resistance, Viral
/
Allergy and Immunology
/
Gag Gene Products, Human Immunodeficiency Virus
/
Pol Gene Products, Human Immunodeficiency Virus
/
Human Immunodeficiency Virus Proteins
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2012
Type:
Article
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