Effect of BYDV-MP nuclear localization signal on the movement of PVX / 病毒学报
Chinese Journal of Virology
; (6): 35-44, 2012.
Article
in Zh
| WPRIM
| ID: wpr-354774
Responsible library:
WPRO
ABSTRACT
Abstract:By using PVX derived vector pGR107, the effect of BYDV-MP nuclear localization signal on the movement of PVX was studied. BYDV-MP was cloned into pGR107 using GFP as an indicator. BYDV-MP was then shown to induce the systemic infection and exacerbate the symptom of PVX through infecting Nicotiana benthamiana. When the PVX gene encoding 25kD protein, which functioned as a systematic movemnet protein,was deleted and the above experiment was repeated, the result showed that BYDV-MP could compensate the systemic movement of PVX. A serial mutants with substitutions on the fifth, sixth and seventh amino acids of BYDV-MP nuclear localization signal was further constructed. It was found that the mutants at the fifth, sixth amino acids in BYDV-MP nuclear localization signal could only delay or weaken systemic movement of PVX whereas the mutant at seventh amino acid could entirely inhibit systemic movement of PVX.
Full text:
1
Index:
WPRIM
Main subject:
Physiology
/
Molecular Sequence Data
/
Chemistry
/
Amino Acid Sequence
/
Potexvirus
/
Luteovirus
/
Nuclear Localization Signals
/
Green Fluorescent Proteins
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Plant Viral Movement Proteins
/
Genetics
Language:
Zh
Journal:
Chinese Journal of Virology
Year:
2012
Type:
Article