Topoisomerase II inhibitors etoposide and doxorubicin induced rearrangement and fusion of AML1 gene / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the relationship between topoisomerase II inhibitors and t(8;21) chromosomal translocation.</p><p><b>METHODS</b>The rearrangements of AML1 and ETO genes were detected by Southern Blot and the AML1-ETO fusion gene by nested RT-PCR combined with sequencing in K562 cells treated with etoposide (Vp16) and doxorubicin (DOX).</p><p><b>RESULTS</b>The rearrangements of AML1 gene were detectable after DOX treatment at concentrations of 10, 50 and 100 micro mol/L for 16 h, AML1-ETO fusion gene appeared after 50 micro mol/L DOX treatment for 48 h.</p><p><b>CONCLUSION</b>Induction of AML1 gene rearrangement and fusion by topoisomerase II inhibitors, represents one of the molecular mechanisms of t(8;21) chromosomal translocation.</p>