Effect of Tpo and/or IL-11 gene modified stromal cells on the expansion of CD34+ CD38- hematopoietic primitive progenitor cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 589-592, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-354819
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Tpo and/or IL-11 gene modified stromal cells on the expansion of CD(34)(+) hematopoietic stem/progenitor cells in cord blood.</p><p><b>METHODS</b>Retroviral vectors containing Tpo or IL-11 gene were constructed and used to transfect the stromal cell line HFCL. Tpo and/or IL-11 mRNA was assayed by Northern blot. Non-modified stromal cells were used, CD(34)(+) hematopoietic stem/progenitor cells from cord blood were expanded on gene-modified stromal cells for 7 days. The phenotype of CD(34)(+)CD(38)(-) primitive progenitors was detected by flow cytometry.</p><p><b>RESULTS</b>HFCL expressed Tpo and/or IL-11 mRNA after transfected by the retroviral vectors. The percentages of CD(34)(+)CD(38)(-) primitive progenitors in the cultures of Tpo, IL-11 and Tpo + IL-11 modified HFCL were (1.8 +/- 0.24)%, (1.62 +/- 0.23)%, and (2.45 +/- 0.28)%, respectively, which were higher than that in the control [(0.8 +/- 0.23)%].</p><p><b>CONCLUSION</b>The stromal cells modified by Tpo and/or IL-11 gene were able to enhance ex vivo expansion of CD(34)(+) and CD(34)(+)CD(38)(-) hematopoietic stem/progenitor cells from cord blood.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Thrombopoietin
/
Hematopoietic Stem Cells
/
Membrane Glycoproteins
/
Antigens, CD
/
Stromal Cells
/
Interleukin-11
/
Antigens, CD34
/
Cell Biology
/
ADP-ribosyl Cyclase
Limits:
Humans
/
Infant, Newborn
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2003
Type:
Article
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