Study on DNA methylation status of WT1 gene promoter in leukemia cell / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 527-529, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-354829
ABSTRACT
<p><b>OBJECTIVE</b>To analyse the WT1 expression and its DNA methylation status of its promoter domain.</p><p><b>METHOD</b>The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.</p><p><b>RESULTS</b>WT1 was overexpressed in HL60, K562 and KG1 leukemia cell lines, but not in U937 and PBMNC. Methylation of WT1 promoter was not observed in HL60 cells.</p><p><b>CONCLUSION</b>DNA methylation of WT1 gene promotor did not inhibit its expression. Other mechanisms may appear to regulate the WT1 expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Leukemia
/
Polymerase Chain Reaction
/
Genes, Wilms Tumor
/
Promoter Regions, Genetic
/
DNA Methylation
/
Cell Line, Tumor
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2003
Type:
Article
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