Identification of differentially expressed genes in Lin-CD34- and Lin-CD34+ cells / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To identify genes that differentially expressed in Lin(-)CD(34)(-) and Lin(-)CD(34)(+) cells.</p><p><b>METHODS</b>With Lin(-)CD(34)(-) cells as tester and Lin(-)CD(34)(+) cells as driver, cDNA subtractive library for Lin(-)CD(34)(-) cells was constructed using suppression subtractive hybridization technique. Part of clones in the library were sequenced and the homologue analysis was conducted against the DNA database in GenBank.</p><p><b>RESULTS</b>593 clones containing an average of 300 - 500 bp insert were identified. Of them, 53 randomly selected ESTs were sequenced. Homologue analysis revealed that 37 ESTs represented 10 known genes, and the other 16 ESTs represented 4 novel sequences.</p><p><b>CONCLUSION</b>Part of specifically expressed genes in Lin(-)CD(34)(-) cells were identified, which maybe related to Lin(-)CD(34)(-) cells' specific characteristics.</p>