Dynamic changes of cytokines in G-CSF mobilized peripheral blood / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 398-401, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-354844
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the level and significance of interleukin-8 (IL-8), soluble intercellular adhesion molecule (sICAM-1) and soluble vascular cell adhesion molecule-1 (sVCAM-1) in patients peripheral blood (PB) during mobilization for peripheral blood stem cells harvesting.</p><p><b>METHODS</b>The levels of IL-8, sICAM-1 and sVCAM-1 in patients were dynamically assayed by ELISA during the mobilization procedure and the number of CD(34)(+) cell, white blood cell (WBC) and platelet (BPC) by flow cytometric analysis and hematometry respectively. Colony formation was assayed by using semisolid methycellulose culture.</p><p><b>RESULTS</b>There was a significant increase in plasma levels of IL-8 and both adhesion molecules [IL-8 (247.4 +/- 84.2) microg/L (P < 0.01); sICAM-1 (530.3 +/- 286.1) microg/L (P = 0.002 7); sVCAM-1 (575.3 +/- 350.4) microg/L (P = 0.001 3)] during the mobilization process; furthermore, IL-8 and sVCAM-1 concentration in the patient's plasma was paralleled to the numbers of CD(34)(+) cell, CFU-GM, WBC and BPC (P < 0.001).</p><p><b>CONCLUSION</b>The levels of IL-8, sICAM-1 and sVCAM-1 in the patient's plasma were correlated to the PB number of CD(34)(+) cells, CFU-GM, WBC and BPC during the mobilization process. It suggested that analysis of IL-8, and sVCAM-1 dynamic changes may serve as markers for CD(34)(+) cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Enzyme-Linked Immunosorbent Assay
/
Granulocyte Colony-Stimulating Factor
/
Interleukin-8
/
Treatment Outcome
/
Intercellular Adhesion Molecule-1
/
Vascular Cell Adhesion Molecule-1
/
Hematopoietic Stem Cell Mobilization
/
Therapeutic Uses
/
Methods
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2003
Type:
Article
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