Effect of T3 on the expression of transferrin receptor and ferritin in K562 cells and its possible mechanism / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 181-184, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-354872
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of T(3) on the expression of transferrin receptor (TfR) and ferritin (Fn) in K562 cells and its possible mechanism.</p><p><b>METHODS</b>Flow cytometry was used for the detection of TfR expression, radioimmunoassay for Fn expression, RNA/protein band shift assay for the binding activity of iron regulatory protein (IRP) and iron responsive elements (IRE), and RT-PCR for TfR and Fn mRNA levels.</p><p><b>RESULTS</b>Different concentration of T(3) significantly increased Fn expression of K562 cells, especially at 100 nmol/L and 200 nmol/L (p < 0.05). However, T(3) had no effect on TfR expression. T(3) decreased the binding activity between IRP and IRE, particularly at concentration of 50 nmol/L. Different concentration of T(3) increased Fn-H mRNA level at different time point while it had no effect on TfR mRNA level.</p><p><b>CONCLUSION</b>T(3) increased Fn expression of K562 cells through the possible mechanisms of either the post-transcriptional regulation or transcriptional modulation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Triiodothyronine
/
Receptors, Transferrin
/
RNA, Messenger
/
Radioimmunoassay
/
Gene Expression Regulation, Leukemic
/
K562 Cells
/
Reverse Transcriptase Polymerase Chain Reaction
/
Ferritins
/
Flow Cytometry
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2003
Type:
Article
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