The Application of RACE Technique to Clone the Full-Length cDNA of A Novel Leukemia Associated Gene LRP16 / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 18-21, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-354989
ABSTRACT
LRP16 is a novel gene which was found in our laboratory by using methylation-sensitive restriction landmark genomic scanning (RLGS) technique. In order to clone the full-length cDNA of this leukemia relapse associated gene, the method of rapid amplification of cDNA end (RACE) was employed. By optimizing some procedures of RACE method, the 5'- and 3'-untranslated region of LRP16 cDNA was successfully sequenced. Then, the full length of LRP16 cDNA and open reading frame (ORF) was constructed and was registered in GenBank. The above-mentioned procedure demonstrated RACE technique is a rapid and sensitive method for cloning unknown gene. Especially, it is very useful to cloning the 5'- and 3'-untranslated region of a novel gene.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2001
Type:
Article
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