Effect of hTERT antisense oligodeoxynucleotide on telomerase activity in bladder cancer cells in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 779-781, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-355019
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of hTERT antisense oligodeoxynucleotide on telomerase activity in bladder cancer cells.</p><p><b>METHODS</b>Antisense phosphorothioate oligodeoxynucleotide (AS PS-ODN) was synthesized and purified. Telomerase activity was measured by telomerase PCR ELISA kit. hTERT mRNA expression was detected by reverse transcription-polymerase chain reaction (RT-PCR), and hTERT protein by immunohistochemistry and flow cytometry.</p><p><b>RESULTS</b>Telomerase activity was decreased in T24 cells 48 h after treatment with AS PS-ODN, and was significantly inhibited at 72 h.</p><p><b>CONCLUSION</b>AS PS-ODN can significantly inhibit telomerase activity by down-regulating hTERT mRNA and protein expression in bladder cancer cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Urinary Bladder Neoplasms
/
RNA, Messenger
/
Gene Expression Regulation, Neoplastic
/
Telomerase
/
Oligodeoxyribonucleotides, Antisense
/
Cell Line, Tumor
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Type:
Article
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