Effect of aging on pulmonary ICAM-1 and MCP-1 expressions in rats with lipopolysaccharide- induced acute lung injury / 南方医科大学学报
Journal of Southern Medical University
;
(12): 584-587, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-355065
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of aging on the expressions of monocyte chemoattractant protein 1 (MCP-1) and intercellular adhesion molecule 1 (ICAM-1) in the lung tissue of rats with lipopolysaccharide (LPS)-induced acute lung injury (ALI).</p><p><b>METHODS</b>Both young (3 months old) and aged (27 months old) female Wistar rats were randomly divided into two groups (n=8), namely the normal control and LPS-induced ALI groups. Immunohistochemistry for of ED-1 was used to detect the infiltrating inflammatory cells. Western blot and Northern blot analyses were employed for evaluating the expressions of MCP-1 and ICAM-1 at the protein and mRNA levels.</p><p><b>RESULTS</b>Virtually no ED-1-positive cells were found in the lung tissue of the control rats in the young and aged groups. After LPS-induced ALI, ED-1-positive cells in the lung tissues increased significantly in both young and aged groups (P<0.05), and the increment was more obviously in the aged group (P<0.05). In the two normal control groups, the aged rats showed significantly higher expressions of MCP-1 and ICAM-1 than the young rats (P<0.05); LPS significantly up-regulated their expression in the young and aged groups (P<0.05), but the latter showed greater increments (P<0.05). The aged rats with ALI also showed significantly greater MCP-1 and ICAM-1 increments than those of the young rats (P<0.05).</p><p><b>CONCLUSIONS</b>Aging may upregulate lung MCP-1 and ICAM-1 expressions and enhance LPS-induced increments of MCP-1 and ICAM-1 expressions to exacerbate the pulmonary inflammation in rats.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aging
/
RNA, Messenger
/
Random Allocation
/
Up-Regulation
/
Lipopolysaccharides
/
Rats, Wistar
/
Intercellular Adhesion Molecule-1
/
Chemokine CCL2
/
Acute Lung Injury
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Type:
Article
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