Effect of recombinant human erythropoietin on bcl-2 protein expression in the retina in a rabbit model of acute high intraocular pressure / 南方医科大学学报
Journal of Southern Medical University
;
(12): 552-554, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-355073
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of recombinant human erythropoietin (rhEPO) on the expression of bcl-2 protein in the retina of rabbits with acute high intraocular pressure and explore the mechanism underlying the protective effect of rhEPO on the retina against ischemia-reperfusion injury.</p><p><b>METHODS</b>rhEPO was injected subcutaneously in the ear of a rabbit model of acute high intraocular pressure induced by physiological saline perfusion into the anterior chamber. Bcl-2 protein expression in the retina of the rabbits was observed by immunohistochemical staining on days 1, 3, 7, and 14 after retinal ischemia-reperfusion and compared with that in normal rabbits and untreated rabbit models.</p><p><b>RESULTS</b>bcl-2-positive cells were observed in the retina of normal rabbits with a mean positive cell number of 10.5-/+1.2 in each high-power visual field. Compared with that in the normal control group, the number of the positive cells decreased significantly in both the model group and EPO group (P<0.05, P<0.01), but the latter group showed a significantly greater number than the former (P<0.05 at day 7 and P<0.01 at day 14).</p><p><b>CONCLUSION</b>Systemic administration of rhEPO can up-regulate the expression of bcl-2 protein in the retina of rabbits with acute high intraocular pressure, which is probably one of the mechanisms for the protective effect of rhEPO on the retina against ischemia-reperfusion injury.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Retina
/
Recombinant Proteins
/
Random Allocation
/
Ocular Hypertension
/
Erythropoietin
/
Proto-Oncogene Proteins c-bcl-2
/
Therapeutic Uses
/
Drug Therapy
/
Metabolism
Type of study:
Controlled clinical trial
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Type:
Article
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