Neuronal differentiation of PC12 cells induced by human periodontal ligament fibroblast-conditioned medium / 南方医科大学学报
Journal of Southern Medical University
; (12): 549-551, 2010.
Article
in Zh
| WPRIM
| ID: wpr-355075
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To examine whether human periodontal ligament fibroblasts (hPDLF) express neural growth factor (NGF).</p><p><b>METHODS</b>PC12 cells were cultured in hPDLF-conditioned medium to evaluate the secretion of NGF by hPDLF.</p><p><b>RESULTS</b>hPDLF-conditioned medium resulted in neuronal differentiation and neurite outgrowth from PC12 cells. Only a small percentage of the cells grown in anti-NGF antiserum and DMEM expressed neuritis.</p><p><b>CONCLUSION</b>hPDLF are capable of synthesizing NGF and secreting it into the medium as the signaling molecules for innervation during the healing of the periodontal tissue.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Periodontal Ligament
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Pharmacology
/
Pheochromocytoma
/
Periodontium
/
Cell Differentiation
/
Cells, Cultured
/
PC12 Cells
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Culture Media, Conditioned
/
Cell Biology
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2010
Type:
Article