Inhibitory effect of ZD6474 combined with adriamycin on MCF-7 human breast cancer cells in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 523-525, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-355084
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the killing effect of ZD6474 combined with adriamycin (ADM) on MCF-7 human breast cancer cells.</p><p><b>METHODS</b>The inhibitory effects of ZD6474 and ADM alone and in combination on the proliferation of MCF-7 cells were assessed by MTT assay. The cell cycle and cell apoptosis were detected by flow cytometry.</p><p><b>RESULTS</b>ZD6474 and ADM both significantly inhibited the proliferation of MCF-7 cells, showing a synergistic effect of their reactions in combined use (P<0.05). ZD6474 or ADM alone caused cell cycle arrest at G0/G1 and S phases, respectively. Combined use of the two drugs resulted in significant reduction of the M-phase cell percentage and cell cycle arrest at G0/G1 and S phases. The coadministration of the drugs significantly increased the apoptosis rate of the cells as compared with ZD6474 or ADM treatment alone (P<0.05).</p><p><b>CONCLUSIONS</b>ZD6474 and ADM show a synergistic effect in inhibiting the proliferation and inducing apoptosis of MCF-7 cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Piperidines
/
Quinazolines
/
Breast Neoplasms
/
Doxorubicin
/
Cell Cycle
/
Apoptosis
/
Cell Line, Tumor
/
Cell Proliferation
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Type:
Article
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