Modification of HPV type 16 E6 and E7 genes, and analysis of stability and immunogenicity of the modified proteins / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 124-127, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-355113
ABSTRACT
<p><b>BACKGROUND</b>To select the mutants of HPV type 16 E6 and E7 genes suitable for construction of vaccine for treatment of cervical cancer.</p><p><b>METHODS</b>E6 and E7 genes were modified by site-directed mutagenesis. Several recombinant vaccina viruses were constructed by inserting the E6 or E7 mutants into the genome of vaccina virus Tiantan strain and employed to study their antigenicity.</p><p><b>RESULTS</b>Western blot assay showed that the E6 ?mutant? with substitution of Gly for Leu at amino acid site 50 and E7 mutant with substitution of Gly for Cys-24 and Glu-26 had no effect on their stability and antigenicity, but change of the Cys at position 91 of E7 dramatically reduced its stability and antigencity. Conclusion The results confirmed that the Zinc-finger structure at the E7 C-terminal? plays an important role in the integrity and stability of E7 protein.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Papillomaviridae
/
Repressor Proteins
/
Vaccinia virus
/
Oncogene Proteins, Viral
/
Mutagenesis, Insertional
/
Zinc Fingers
/
Allergy and Immunology
/
Papillomavirus E7 Proteins
/
Genetics
/
Mice, Inbred C57BL
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2002
Type:
Article
Similar
MEDLINE
...
LILACS
LIS