Evaluation of aminoglycoside resistance phenotypes and genotyping of acetyltransferase in Escherichia coli / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 83-86, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-355141
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the prevalence of aminoglycoside resistance and genotyping of acetyltransferase in Escherichia coli.</p><p><b>METHODS</b>Resistance phenotypes to 12 antibiotics of 44 Escherichia coli isolates were analyzed using agar dilution method and 3 aminoglycoside resistance genes aac(3)-I, II and aac(6')-I were determined by PCR method.</p><p><b>RESULTS</b>In 44 clinical isolates, the occurrence of ESBLs was 45.45%, resistance rates were discrepant for amikacin (18.18%), gentamicin (56.82%) and tobramycin (61.36%), the prevalence of phenotype TG (tobramycin and gentamicin) indicative of aac(3)-II production and TGA (tobramycin, gentamicin and amikacin) indicative of aac(6')-I production were 36.36% and 18.18%, respectively. The most common aminoglycoside resistance genotype of acetyltransferase was aac(3)-II (52.27%) and aac(6')-I was lower (29.55%), but no aac(3)-I was detected.</p><p><b>CONCLUSION</b>At least 2 acetyltransferase genes exist in this area i.e. aac(3)-II and aac(6')-I.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phenotype
/
Tobramycin
/
Acyltransferases
/
Amikacin
/
Gentamicins
/
Drug Resistance, Bacterial
/
Escherichia coli
/
Genetics
/
Genotype
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2006
Type:
Article
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