Activity of the TNF-related apoptosis-inducing ligand gene expressed from the hTERT promoter on colon cancer cell line HT-29 / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 45-49, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-355147
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the expression and activity of TNF-related apoptosis-inducing ligand (TRAIL) gene expressed from the hTERT promoter on colon cancer cell line HT-29.</p><p><b>METHODS</b>GFP/TRAIL gene expressed from the hTERT promoter was transfected into HT-29 with adenoviral vectors system, expression and apoptosis inducing ability of GFP/TRAIL protein were determined with fluorescence-activated cell sorting (FACS) method.</p><p><b>RESULTS</b>The expression of GFP gene was 31.4 % and 67.0 % with either hTERT promoter or CMV promoter in DLD1 cells; GFP/TRAIL gene was able to inhibit cell growth (74.2%) and induce apoptosis (25.8%) of HT-29 cells. There was significant difference between Ad/hTERT-gTRAIL and the other two control groups (PBS and Ad/CMV-GFP, P<0.05).</p><p><b>CONCLUSION</b>The GFP/TRAIL gene with hTERT promoter transfected by adenoviral vector was successfully expressed in HT-29 cell, which can both inhibit cell growth and induce apoptosis of colon cancer cell line HT-29.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Tumor Cells, Cultured
/
Adenoviridae
/
Promoter Regions, Genetic
/
Apoptosis
/
Colonic Neoplasms
/
Telomerase
/
HT29 Cells
/
Green Fluorescent Proteins
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2006
Type:
Article
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