Mycophenolic acid induced apoptosis in T lymphocytic cells Molt-4 and its mechanism / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 339-343, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-355210
ABSTRACT
<p><b>OBJECTIVE</b>To determine whether mycophenolic acid (MPA) exerts an apoptotic effect on human leukemic T cells Molt-4 and to elucidate its possible mechanism.</p><p><b>METHODS</b>Cell morphology, DNA fragmentation, cell cycle,percentage of annexin V positive cells and enzymatic activity of caspase-3 were measured by microscopic, electrophoretic and flow cytometric techniques respectively. Human leukemic B cell line Raji was used as control.</p><p><b>RESULT</b>MPA could induce apoptosis of Molt-4 cells with dose-and time-dependent manners; cells were blocked in the S phase. The activity of caspase-3 was enhanced in a dose-dependent manner in Molt-4 cells treated with MPA for 24 h. MPA could not induce apoptosis in Raji cells.</p><p><b>CONCLUSION</b>MPA can induce apoptosis in Molt-4 cells which may be involved in the activation of caspase-3.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Time Factors
/
Tumor Cells, Cultured
/
Leukemia, T-Cell
/
Apoptosis
/
Caspases
/
Dose-Response Relationship, Drug
/
Caspase 3
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2005
Type:
Article
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