Application of DHPLC in human genomic SNP screening and genotyping for all-trans-retinol dehydrogenase / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 248-254, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-355231
ABSTRACT
<p><b>OBJECTIVE</b>To assess the efficacy of denaturing high-performance liquid chromatography (DHPLC) as a new screening assay for sequence variation in SNP detecting and new approach to SNP genotyping.</p><p><b>METHODS</b>DHPLC was used to screen SNPs in 8 DNA pools each consisting of DNA from 5 individuals, and genotype the identified SNPs in 150 Chinese subjects from Hong Kong.</p><p><b>RESULTS</b>Seventeen SNPs were identified 12 were novel and 5 were previously reported; 11 were found in screening stage and the other 6 were found in genotyping stage; 2 were only found in Caucasian samples; 3 showed ethnic difference of minor allele frequency(MAF); 7 were common with the MAF>0.05 in Chinese samples.</p><p><b>CONCLUSION</b>The results demonstrated the efficiency of DHPLC in screening SNPs when coupled with DNA pooling strategy, and in genotyping SNPs.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Chromatography, High Pressure Liquid
/
Sequence Analysis, DNA
/
Point Mutation
/
Polymorphism, Single Nucleotide
/
Alcohol Oxidoreductases
/
Genetics
/
Genotype
/
Methods
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2005
Type:
Article
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