Study on lysosomes degradation of ricin A chain / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 212-216, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-355239
ABSTRACT
<p><b>OBJECTIVE</b>To study lysosomes involvement in the degradation of ricin A chain.</p><p><b>METHODS</b>A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain (rRTA) and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B. The cytotoxicity of recombinant proteins was measured by the MTT method.</p><p><b>RESULTS</b>Recombinant RTA-KFERQ was 49.87%, 54.18% and 88.68% less cytotoxic than RTA itself on the three cell lines HEPG2, Hela and A549, respectively.</p><p><b>CONCLUSION</b>Lysosomes can degrade, but not completely inactivate RTA in different cells, suggesting cells may have other degradation pathways for RTA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Ricin
/
Recombinant Proteins
/
Tumor Cells, Cultured
/
HeLa Cells
/
Chromatography, Affinity
/
Escherichia coli
/
Genetics
/
Lung Neoplasms
/
Lysosomes
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2005
Type:
Article
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