Effect of RNA interference of CD59 gene on proliferation of non-small cell lung cancer cell line GLC-P in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 903-906, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-355260
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of CD59 gene inhibition mediated by RNA interference on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) GLC-P cells in vitro.</p><p><b>METHODS</b>Recombinant plasmids for RNA interference of CD59 gene were constructed and transfected into GLC-P cells via lipofectamine 2000. The stably transfected cells were examined with real-time RT-PCR, MTT assay and enzyme-linked immunosorbent assay to investigate the changes in cell proliferation and apoptosis.</p><p><b>RESULTS</b>Compared with the control cells, the cells transfected with CD59-siRNA showed significantly decreased expression levels of CD59 mRNA (P<0.05) and significantly inhibited cell proliferation.</p><p><b>CONCLUSION</b>CD59 gene is highly expressed in NSCLC and RNA interference-mediated CD59 silencing can strongly inhibit the proliferation and induce apoptosis in GLC-P cells, which shed light on a potentially new target for targeted gene therapy of NSCLC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
RNA, Messenger
/
Enzyme-Linked Immunosorbent Assay
/
Transfection
/
Genetic Therapy
/
Apoptosis
/
Carcinoma, Non-Small-Cell Lung
/
CD59 Antigens
/
RNA, Small Interfering
/
RNA Interference
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2015
Type:
Article
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