Protective actions of Rubus coreanus ethanol extract on collagenous extracellular matrix in ultraviolet-B irradiation-induced human dermal fibroblasts
Nutrition Research and Practice
;
: 279-284, 2007.
Article
in English
| WPRIM
| ID: wpr-35550
ABSTRACT
Solar ultraviolet (UV) irradiation leads to distinct changes in the skin connective tissues by degradation of collagen, which is a major structural component in the extracellular matrix. UV irradiation induces the production of matrix metalloproteinases (MMP) capable of attacking native fibrillar collagen and responsible for inhibiting the construction of collagenous extracellular matrix. In this study, we attempted to investigate the protective actions of Rubus coreanus ethanol extract (RCE) on the MMP production and the consequent procollagen/collagen degradation in UV-B-irradiated human dermal fibroblasts. The analytical data showed that Rubus coreanus ethanol extract was mostly comprised of cyanidin 3-rutinoside. Pre-treatment of fibroblasts with this extract inhibited UV-B-induced production of MMP-1, MMP-8 and MMP-13 in dose-dependent manners. In addition, Western blot analysis and immunocytochemical staining assay revealed that RCE markedly augmented the cellular levels of procollagen/collagen declined in UV-B-exposed dermal fibroblasts. These results demonstrate that RCE blocks UV-B-induced increase of the collagen degradation by inhibiting MMP production. Thus, RCE may act as an agent inhibiting excessive dermal collagen degradation leading to the skin photoaging.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Blotting, Western
/
Collagen
/
Connective Tissue
/
Matrix Metalloproteinases
/
Fibrillar Collagens
/
Ethanol
/
Extracellular Matrix
/
Fibroblasts
Limits:
Humans
Language:
English
Journal:
Nutrition Research and Practice
Year:
2007
Type:
Article
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