Effects end mechanisms of curcumol beta-cyclodextrin compound on the proliferation and apoptosls of esophageal carcinoma cell line TE-1 / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 85-89, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-355584
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects and mechanisms of Curcumol beta-cyclodextrin Compound (CbetaC) on the proliferation and apoptosis of esophageal carcinoma cell line TE-1.</p><p><b>METHODS</b>The CbetaC was prepared by saturated solution and confirmed by infrared absorption spectroscopy. The effects of CbetaC (at 25, 50, 100 mg/L) on the proliferation of human esophageal carcinoma cell line TE-1 in vitro was analyzed by MTT assay. The cell cycles and apoptosis were detected by flow cytometer. The relative expression of survivin mRNA was detected by real-time fluorescent quantitative PCR and calculated by the 2(-deltaCt) method. The protein expression of survivin was measured by Western blot.</p><p><b>RESULTS</b>Compared with the control group, results of MTT showed that CbetaC at each dose significantly inhibited the proliferation of TE-1 cells in a dose-dependent manner (P < 0.05). The results of flow cytometry showed that CbetaC resulted in the cell cycle arrest at G0/G1 and G2/M phase, and promoted the cell apoptosis. Besides, when compared with the control group, the protein and mRNA expressions of survivin obviously decreased in each CbetaC group (P < 0.05).</p><p><b>CONCLUSIONS</b>CbetaC could inhibit the proliferation of esophageal carcinoma cell TE-1 and promote the apoptosis. Its inhibition on the survivin expression was correlated with its inhibition on the malignant phenotypes of esophageal carcinoma cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Sesquiterpenes
/
Esophageal Neoplasms
/
Apoptosis
/
Cell Line, Tumor
/
Beta-Cyclodextrins
/
Cell Proliferation
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2013
Type:
Article
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