Modulation effects of human immature and mature dendritic cells on glatiramer acetate specific T cell lines in vitro / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 409-415, 2003.
Article
in English
| WPRIM
| ID: wpr-355635
ABSTRACT
A large body of evidence demonstrates that dendritic cells (DC) play a pivotal role in the control of immunity by priming and tolerizing T cells. In multiple sclerosis (MS), autoreactive T cells are proposed to play a pathogenic role by secreting pro-inflammatory cytokines, but comparison studies on the effects of immature and mature dendritic cells on the cytokines profile of antigen-specific T cell lines are lacking. To evaluate the actions of dendritic cell maturation on T cell polarization, the effects of immature and mature dendritic cells derived from MS patients on in vitro proliferative responses, and cytokine production by glatiramer acetate (GA)- specific T cell lines (TCL) derived from MS patients were analyzed. The results demonstrated that it is easy to derive GA-specific TCL from MS patients with high specificity; lipopolysaccharide can efficiently induce DC maturation within 24 hours at a concentration of 5 micro g/ml; mature DC showed higher co-stimulatory capacity of GA-specific TCLs than immature DC. GA-specific TCLs produce dominantly IL-2, IL-4, IFN-gamma and IL-10, but low levels of IL-6. In contrast to immature DC, mature DC enhanced capacity to induce IL-6 and IL-10 secretion, but down-regulate IL-2, IL-4 and IFN-gamma production by GA- specific TCLs. It is concluded that DC maturation status modulating proliferation of TCL and production of cytokines may represent another focus for the study on both immuno-pathogenesis and immunotherapeutic interventions in MS.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Physiology
/
Dendritic Cells
/
Lymphocyte Activation
/
T-Lymphocytes
/
Cell Line
/
Cytokines
/
Allergy and Immunology
/
Glatiramer Acetate
/
Multiple Sclerosis
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
English
Journal:
Journal of Experimental Hematology
Year:
2003
Type:
Article
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