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Development of the human/rat chimera model with neonatal rats / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 297-300, 2003.
Article in Chinese | WPRIM | ID: wpr-355660
ABSTRACT
The purpose of this study was to transplant neonatal rat with human cord blood Lin(-) cells to test the possibility of this xenograft model. The Lin(-) cells were purified from human cord blood (CB) using negative selection strategy based on different lineage-specific antigens. The Lin(-) cells were injected into the liver of neonatal rats using a microinjector at an average of 5 x 10(5) cells for each. Peripheral blood (PB) and spleen were collected at 2,4 and 8 weeks after injection. Flow cytometry was performed to detect human cells in the rat PB, PCR was used to detect human cells in PB as well as spleen. The results showed that a definite proportion of human cells existed in peripheral blood of chimeric rat and the human specific beta2 microglobulin gene fragments were detected in spleen genomic DNA of chimeric rat. It is concluded that human/rat chimera model can be developed with neonatal rats. Human/rat xenograft model may provide a useful and convenient method for human hematopoietic stem cell assay in vivo.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Spleen / Transplantation, Heterologous / Blood / DNA / Polymerase Chain Reaction / Beta 2-Microglobulin / Rats, Sprague-Dawley / Leukocyte Common Antigens / Transplantation Chimera / Cord Blood Stem Cell Transplantation Limits: Animals / Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Spleen / Transplantation, Heterologous / Blood / DNA / Polymerase Chain Reaction / Beta 2-Microglobulin / Rats, Sprague-Dawley / Leukocyte Common Antigens / Transplantation Chimera / Cord Blood Stem Cell Transplantation Limits: Animals / Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2003 Type: Article