Application of double immunomagnetic positive sorting to ex vivo expansion of marrow CD34(+)CD59(+) cells from patients with paroxysmal nocturnal hemoglobinuria / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 179-183, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-355690
ABSTRACT
Since flow cytometry was not feasible for sorting a huge amount of cells for clinical use, the method of double immunomagnetic positive sorting was used for selection of CD34(+)CD59(+) cells from bone marrow mononuclear cells in patients with paroxysmal nocturnal hemoglobinuria (PNH), which laid the groundwork for clinical ABMT/APBSCT of patients with PNH. Immunomagnetic positive selection was used for two times, the microbeads were removed from the CD34(+) cells selected firstly by means of overnight culture, then the sufficient CD34(+)CD59(+) cells were used for ex vivo expansion. The results showed that the survival, proliferation and colony-forming units of the selected CD34(+)CD59(+) cells by double immunomagnetic positive sorting had no significant difference as compared with that of CD34(+)CD59(+) cells selected by flow cytometry technique. It is suggested that the double immunomagnetic positive sorting promotes the use for separation and purification hematopoietic stem/progenitor cells and other cells with double or multiple markers cells for autologous hematopoietic stem cell transplantation in PNH patients.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Transplantation, Autologous
/
Bone Marrow Cells
/
Immunomagnetic Separation
/
Hematopoietic Stem Cell Transplantation
/
CD59 Antigens
/
Antigens, CD34
/
Cell Biology
/
Flow Cytometry
/
Hemoglobinuria, Paroxysmal
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2003
Type:
Article
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