Synthesis and function analysis of a new thrombopoietin (TPO) mimic peptide / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 128-131, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-355700
ABSTRACT
In order to find a new TPO-mimic peptide with similar activity to TPO while reducing the side effects, a TPO-mimic peptide (P1) screened from a random peptide library was restructured. The new structure of the TPO mimic peptide (P2) was synthesized. After coupling P2 with Dextran 10 and performing intermolecular oxidation, dextran-coupled and dimerized form of P2 were obtained, naming D-P2 and (P2)(2) respectively. The activities of the peptides in vitro were measured with MTT method. The results showed that the EC50 of P2 was 20 nmol/L, 700 times higher than P1. The EC50 of D-P2 and (P2)(2) were 0.35 nmol/L and 0.14 nmol/L, respectively. After administrating to the mouse, the peptides increased the number of platelets in the blood circulation obviously without influence on other blood cells. In conclusion, the TPO-mimic peptides have prospects in treating diseases related with thrombocytopenia.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Platelet Count
/
Thrombopoietin
Limits:
Animals
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2003
Type:
Article
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