Increased expression of transient receptor potential melastatin 7 in mouse cardiac fibroblasts post myocardial infarction / 中华心血管病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe transient receptor potential melastatin 7-like (TRPM7L) expression changes post myocardial infarction (MI) in mouse cardiac fibroblast (CF).</p><p><b>METHODS</b>TRPM7 expression and Ca2+ influx in CF from MI and control mice were quantified by mRNA RT-PCR and whole cell patch clamp technique.</p><p><b>RESULTS</b>(1) TRPM7 expression was significantly upregulated post MI and Ca2+ influx of CF were significantly increased post MI [(7.4 +/- 0.7) pA/pF vs. (16.2 +/- 1.7) pA/pF, P < 0.01] and Ca2+ influx of CF increased 3-fold under lower pH condition; (2) These effects could be blocked by knock-out TRPM7 gene with SiRNA.</p><p><b>CONCLUSION</b>TRPM7L upregulation post MI and under lower pH condition are responsible for increased Ca2+ influx in CF.</p>