Dynamic changes of Smac/DIABLO protein expression in rat donor myocardium suffered from hypothermic preservation / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 296-300, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-356271
ABSTRACT
<p><b>AIM</b>To investigate the effect of different duration of hypothermic preservation on the expression of Smac/DIABLO protein in rat hearts.</p><p><b>METHODS</b>The Langendorff model of isolated rat heart was used. After stored in 4 degrees C Celsior solution for different time (0, 3, 6, 9 or 12 h), the activity of SOD and the content of MDA in heart mitochondria were measured. Cell apoptosis was detected by TUNEL technique. The expression of Smac/DIABLO protein was also analyzed by Western blotting.</p><p><b>RESULTS</b>(1) After hypothermic preservation, the activity of SOD was decreased and the content of MDA was increased in rat hearts in a time-dependent manner. (2) Prolonged the hypothermic preservation, the percentage of apoptotic cell also enhanced. (3) After long-term of cold preservation, the expression of Smac/DIABLO protein increased at 3-6 h of preservation but decreased after 9 h.</p><p><b>CONCLUSION</b>Prolonged the hypothermic preservation might lead to the expression of Smac/DIABLO protein and induce cardiomyocytes apoptosis, which may in turn result in malfunction of cardiomyocytes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Preservation
/
Carrier Proteins
/
Random Allocation
/
Heart Transplantation
/
Rats, Sprague-Dawley
/
Cold Temperature
/
Mitochondrial Proteins
/
Heart
/
Metabolism
/
Methods
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2009
Type:
Article
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