The proliferation profile of mouse spermatoganial stem cells in three types of culture media / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 286-288, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-356274
ABSTRACT
<p><b>AIM</b>To establish an in vitro long-term culture system of mouse Spermatogonial stem cells (SSCs).</p><p><b>METHODS</b>Three types of serum-free culture media, namely, DMEM/F12, KSR (KnockoutM Serum Replacement) and StemPro-34 SFM, to which the same growth factors including GDNF, soluble GFRalpha1 and bFGF were added equally, and MEF(mouse embryonic fibroblast) feeder layer were used to culture mouse SSCs enriched from pup mice testes through differential adherence selection. The activity of stem cells was examined morphologically, and the marker gene expression of SSCs was detected by RT-PCR and immunocytochemical analysis.</p><p><b>RESULTS</b>The activity of SSCs cultured in DMEM/F12 and KSR serum-free media was only maintained for 6-7 days. However, the StemPro-34 SFM medium could maintain the proliferation of cultured SSCs nearly one month.</p><p><b>CONCLUSION</b>StemPro-34 SFM serum-free medium sustains the proliferation of mouse SSCs in vitro.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spermatogonia
/
Stem Cells
/
Cells, Cultured
/
Chemistry
/
Culture Media, Serum-Free
/
Cell Biology
/
Cell Proliferation
/
Mice, Inbred DBA
/
Mice, Inbred ICR
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS