Proteomic analysis of myocardial hypertrophy induced by left kidney artery coarctation in rats / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 237-242, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-356287
ABSTRACT
<p><b>AIM</b>To identify the expression of proteins in cardiomyocytes in rats with left kidney artery coarctation.</p><p><b>METHODS</b>16 male SD rats were separated into 2 groups (n=8) 2 kidney 1 Clip group (2K1C) and sham operation group (SO). The postoperational 8th week, after examination by normal doppler and tissue doppler echocardiography, the extracted proteins from cardiomyocytes were isolated by two-dimensional gel electrophoresis with staining. The gel images were acquired by scanner and 2-DE analysis software. Different spots observed on two 2D gels were selected and identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS).</p><p><b>RESULTS</b>Overall, 21 protein spots showed significant difference, and 14 out of which were identified.</p><p><b>CONCLUSION</b>Kidney artery coactation-induced cardiac hypertrophy displays different expression of proteins in cardiomyocytes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Renal Artery
/
Random Allocation
/
Rats, Sprague-Dawley
/
Cardiomegaly
/
Constriction
/
Proteome
/
Proteomics
/
Metabolism
/
Methods
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2009
Type:
Article
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