Establishment of mouse SP2/0 cell line stably expressing bcr-abl fusion gene fragment / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 601-604, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-356506
ABSTRACT
To establish SP2/0 cell line H-2(d) stably expressing bcr-abl fusion gene fragment, the bcr-abl fusion gene was subcloned into retroviral vector pLXSN from pGEMbcr-abl. The recombinant retroviral vector pLXSNbcr-abl was transfected into PT67 packaging cells with the help of lipofectamine. The positive clones were selected out and cultured after G418 selection. Then viral supernatant was collected to determine viral titer, the viral titer was 2 x 10(7) CFU/ml. The SP2/0 cells were infected with the collected viral supernatant. The results showed that after G418 selection, the bcr-abl fusion gene was integrated into the chromosome of SP2/0 cells infected stably, with recombinant retrovirus and expressed in SP2/0 cells confirmed by PCR and RT-PCR respectively. In conclusion, the mouse tumor cell lines expressing the bcr-abl fusion protein were successfully established and would be used as a experimental cell model for anti-CML immunotherapy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Peptide Fragments
/
Retroviridae
/
RNA, Messenger
/
Transfection
/
Gene Expression Regulation, Neoplastic
/
Cell Line
/
Fusion Proteins, bcr-abl
/
K562 Cells
/
Reverse Transcriptase Polymerase Chain Reaction
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article
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